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Journal: Nature Physics
Article Title: Boundary-guided cell alignment drives mouse epiblast maturation
doi: 10.1038/s41567-026-03176-9
Figure Lengend Snippet: ( A ) Box plots of EPI cell number, showing median (centre), 25th–75th percentiles (box), with whiskers extending to 1.5x interquartile range, with individual embryos shown as dots. Sample sizes: Lamc1 +/+ , n = 9 (E4.5), 13 (E4.75), 11 (E5.0); Lamc1 +/− , n = 12, 28, 20; Lamc1 −/− , n = 4, 11, 9 embryos from 5, 7, and 5 independent Lamc1 +/− × Lamc1 +/− litters. One-way ANOVA with Tukey’s post hoc test, ns = not significant. ( B ) Box plots of EPI cell number, as in ( A ). Sample sizes: Itgb1 +/+ , n = 5 (E4.5), 13 (E4.75), 9 (E5.0); Itgb1 +/− , n = 13, 28, 26; Itgb1 −/− , n = 8, 14, 11 embryos from 5, 8, and 7 independent Itgb1 +/− × Itgb1 +/− litters. One-way ANOVA with Tukey’s post hoc test. *p < 0.05, **p < 0.01, ns = not significant. At E5.0, Itgb1 +/+ vs Itgb1 −/− , p = 0.017; Itgb1 +/− vs Itgb1 −/− , p = 0.001. ( C ) Immunofluorescence images of representative Lamc1 +/+ or +/− and Lamc1 −/− embryos at E5.25, stained for Oct3/4 (EPI, cyan), phalloidin (yellow), and podocalyxin (magenta). n = 28 ( Lamc1 +/+ or +/− ), 6 ( Lamc1 −/− ) embryos from 4 independent Lamc1 +/− × Lamc1 +/− litters. ( D ) Immunofluorescence images of representative Itgb1 +/+ or +/− and Itgb1 −/− embryos at E5.25, stained for Oct3/4 (EPI, cyan), pan-laminin (green), and phalloidin (yellow). n = 13 ( Itgb1 +/+ or +/− ), 2 ( Itgb1 −/− ) embryos from 2 independent Itgb1 +/− × Itgb1 +/− litters. ( E ) Long axis length measurement, shown as violin plots with individual data points. Dot colours indicate embryo stage; red bars show medians. Sample sizes: Lamc1 +/+ , n = 163 cells from 6 embryos (E4.5), 397 from 8 (E4.75), 571 from 10 (E5.0); Lamc1 +/− , n = 199 from 7, 583 from 14, 637 from 10; Lamc1 − / − , n = 75 from 3, 277 from 7, 462 from 9. Mann-Whitney U test (two-sided) without correction for multiple comparisons, each group compared to the reference group [15-29 cells]. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant. Exact p-values: Lamc1 +/+ [30–44], p = 0.345; [45–59], p = 0.189; [60–74], p = 0.236; [75–89], p = 8.72 × 10 −9 . Lamc1 +/− [30–44], p = 0.051; [45–59], p = 1.00 × 10 −3 ; [60–74], p = 5.02 × 10 −6 ; [75–89], p = 7.42 × 10 −6 . Lamc1 −/− [30–44], p = 0.539; [45–59], p = 4.07 × 10 −4 ; [60–74], p = 0.059. ( F ) Long axis length measurement, as in ( E ). Sample sizes: Itgb1 +/+ , n = 110 cells from 3 embryos (E4.5), 399 from 8 (E4.75), 251 from 4 (E5.0); Itgb1 +/− , n = 200 from 5, 560 from 11, 618 from 10; Itgb1 −/− , n = 209 from 7, 470 from 11, 360 from 9. Mann-Whitney U test (two-sided) without correction for multiple comparisons, each group compared to the reference group [15-29 cells]. *p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant. Exact p-values: Itgb1 +/+ : [30–44], p = 2.36 × 10 −4 ; [45–59], p = 0.015; [60–74], p = 8.97 × 10 −3 ; [75–89], p = 2.34 × 10 −4 . Itgb1 +/− : [30–44], p = 0.845; [45–59], p = 0.335; [60–74], p = 0.518; [75–89], p = 0.022. Itgb1 −/− : [30–44], p = 5.65 × 10 −4 ; [45–59], p = 0.022; [60–74], p = 3.71 × 10 −4 . Scale bars, 20 µm. See also Fig. .
Article Snippet:
Techniques: Immunofluorescence, Staining, MANN-WHITNEY